Amino acid sequence of a heat‐stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D

FEBS Letters

Takeda, Tae³  Aimoto, Saburo¹  Hara, Saburo²  Shimonishi, Yasutsugu¹  Takao, Toshifumi¹  Hitouji, Tetsuo¹  Takeda, Yoshifumi³  Miwatani, Toshio³ 

[1] Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan;Department of Chemistry, Faculty of Science, Osaka University, Suita, Osaka 565, Japan;Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan

关键词: Enterotoxin;    Isolation;    Amino acid sequence;    FAB mass spectrometry;   

DOI  :  10.1016/0014-5793(83)80469-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A heat-stable enterotoxin produced by a strain of enterotoxigenic Escherichia coli 18D was purified by ion-exchange and reversed-phase high-pressure liquid chromatography. The amino acid sequence of the purified toxin was determined by Edman-degradation and a combination of fast atom bombardment mass spectrometry and carboxypeptidase digestion to be Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr.

【 授权许可】

Unknown