The amino‐terminal structure of oat phytochrome

FEBS Letters

Kellermann, Josef²  Rüdiger, Wolfhart¹  Schäfer, Wolfram²  Grimm, Rudolf¹ 

[1] Botanisches Institut der Universität München, 8000 München 19, FRG;Max Planck-Institut für Biochemie-Genzentrum, 8033 Martinsried bei München, FRG

关键词: FAB mass spectrometry;    Proteolysis;    HPLC;    Peptide sequencing;    Trypsin;    (Avena sativa L.);   

DOI  :  10.1016/0014-5793(88)80146-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phytochrome from etiolated oat seedlings was digested under carefully controlled conditions with trypsin. A dodecapeptide which contains the blocked amino-terminus of phytochrome was isolated from the digestion mixture and analyzed. According to amino acid analysis and the results obtained in FAB mass spectrometry, the sequence of mature phytochrome starts with N-acetylserine, corresponding to serine-2 derived from the DNA sequence.

【 授权许可】

Unknown