The amino acid sequence of the enterotoxin from Clostridium perfringens type A

FEBS Letters

Granum, Per Einar²  Richardson, Michael¹ 

[1] Department of Botany, University of Durham, Durham DH1 3LE, England;Norwegian Food Research Institute, N-1432 Ås-NLH, Norway

关键词: Clostridium perfringens;    Enterotoxin;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(85)80358-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino acid sequence of the enterotoxin from Clostridium perfringens type A was determined by analysis of peptides derived from the protein by digestion with trypsin chymotrypsin, thermolysin, pepsin, a lysinespecific protease, S. aureus V8 protease and a proline-specific protease, and fragments generated by cleavage with cyanogen bromide or by dilute acetic acid in 7 M guanidine HCl. The sequence which is complete except for the definite order of 3 small peptides between residues 88 and 103 consists of 309 amino acids and contains a correction to our preliminary announcement [(1984) FEMS Symp. 24, 329-330].

【 授权许可】

Unknown