The TRH‐related peptide pyroglutamylglutamylprolinamide is present in human semen

FEBS Letters

Smyth, Derek G.¹  Cockle, Sheena M.¹  Morrell, Jane M.¹  Aitken, Alastair¹  Beg, Fatima¹ 

[1] National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, England

关键词: Semen;    Thyrotrophin-releasing hormone;    Pyroglutamylglutanylprolinamide;    FAB mass spectrometry;    (Human);   

DOI  :  10.1016/0014-5793(89)80900-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently identified a novel peptide in the rabbit prostate complex which cross-reacts with an antibody to thyrotrophin-releasing hormone (TRH) and has the structure pGlu-Glu-ProNH₂. In the present study, high concentrations of a TRH-related tripeptide and also a polypeptide (10–12 kDa) containing a TRH-immunoreactive peptide at its C-terminus were detected in human semen. The low molecular mass TRH-like peptide and the immunoreactive fragment from the polypeptide were isolated from human semen and shown to have identical structures. Amino acid analysis suggested compositions Glx₂, Pro₁, and after mild acid hydrolysis, the same sequence, Glu-Glu-Pro, was established for the two peptides. Fast atom bombardment (FAB) mass spectrometry yielded a pseudomolecular ion (M + H)⁺ of 355.38 which was identical to that of the synthetic peptide pGlu-Glu-ProNH₂. The data demonstrate that human semen contains the TRH-like peptide pyroglutamylglutamylprolinamide and also a polypeptide terminating in the sequence Gln-Glu-ProNH₂.

【 授权许可】

Unknown