Arginine activation of N‐acetylglutamate synthetase in mouse liver

FEBS Letters

Kawamoto, Susumu¹  Tatibana, Masamiti¹ 

[1] Department of Biochemistry, Chiba University School of Medicine, Inohana, Chiba 280, Japan

关键词: Mitochondria;    Urea synthesis;    Acetylglutamate synthetase;    Arginine;    Cycloheximide;    Carbamoyl-phosphate synthetase I;    acetylglutamate;    N-acetyl-L-glutamate;   

DOI  :  10.1016/0014-5793(83)80355-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-Acetyl-L-glutamate sythetase catalyzes the synthesis of N-acetyl-L-glutamate, an allosteric and esential activator of carbamoyl-phosphate synthetase I in the liver of ureotelic animals. The enzyme is activated specifically by arginine. We report here that the sensitivity of the synthetase to activation by arginine increases markedly after intraperitoneal administration to mice of inhibitors of nucleic acid and protein synthesis, including actinomycin D, aurintricarboxylic acid, cycloheximide, emetine and puromycin. The effects of cycloheximide were investigated in detail and an amino acid analysis was made of the homogenate of freeze-clamped livers of control or cycloheximide-treated mice.

【 授权许可】

Unknown