The effect of dichloroacetate and hydroxypyruvate on the entry of 14C from [1‐14C]alanine into urea in rat hepatocytes

FEBS Letters

Hems, Reginald¹ 

[1] Metabolic Research Laboratory, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, England

关键词: Urea synthesis;    Equilibration of metabolic CO2;    Dichloroacetate;    Hydroxypyruvate;    Mitochondrial pH gradient;    Hepatocyte;   

DOI  :  10.1016/0014-5793(83)80977-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Entry of metabolic ¹⁴CO₂ into urea is shown to be decreased by dichloroacetate although the production of ¹⁴CO₂ is stimulated 2-fold. Hydroxypyruvate, a product of dichloroacetate metabolism, increases the incorporation of metabolic ¹⁴CO₂ into urea. It is proposed that these effects result from changes in the cytoplasmic-mitochondrial pH gradient.

【 授权许可】

Unknown