期刊论文详细信息
FEBS Letters
Equilibration of metabolic CO2 with preformed CO2 and bicarbonate
Hems, Reginald1  Saez, Guillermo T.1 
[1] Metabolic Research Laboratory, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, England
关键词: Urea synthesis;    Equilibration of metabolic CO2;    Carbamoylphosphate synthetase;    Mitochondrial carbonic anhydrase;    Hepatocytes;    Isotope data interpretation;   
DOI  :  10.1016/0014-5793(83)80659-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Entry of metabolic 14CO2 into urea is shown to occur more readily than it equilibrates with the general pool of cellular plus extracellular bicarbonate plus CO2. Since the sites of CO2 production (pyruvate dehydrogenase and oxoglutarate dehydrogenase) and of fixation (carbamoylphosphate synthetase) are intramitochondrial, it is likely that the fixation of CO2 is also more rapid than its equilibration with the cytoplasmic pool of bicarbonate plus CO2. This observation may point to a more general problem concerning the interpretation of isotope data, when compartmentation or proximity of sites of production and utilisation of metabolites may result in the isotope following a preferred pathway.

【 授权许可】

Unknown   

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