Valinomycin can depolarize mitochondria in intact lymphocytes without increasing plasma membrane potassium fluxes

FEBS Letters

Brand, Martin D.¹  Felber, Suzanne M.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Valinomycin;    Mitochondria;    Plasma membrane;    Potassium flux;    Membrane potential;    Lymphocyte;    TPMP+;    methyltriphenylphosphonium cation;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethane-sulphonic acid;   

DOI  :  10.1016/0014-5793(82)81317-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have simultaneously measured [³H]methyltriphenylphosphonium⁺ and ⁸⁶Rb⁺ distribution across the mitochondrial and plasma membranes of pig mesenteric lymphocytes. At low concentrations the K⁺ ionophore valinomycin depolarizes the mitochondria but does not increase Rb⁺ fluxes across the plasma membrane. At high concentrations of valinomycin the plasma membrane becomes permeable to Rb⁺ and both TPMP⁺ and Rb⁺ distribution give a measure of the K⁺ diffusion potential.

【 授权许可】

Unknown