Expression of mRNAs encoding the α1 and the β1 subunits of Na+,K+‐ATPase in human lymphocytes activated with phytohaemagglutinine

FEBS Letters

Marakhova, I.I.¹  Osipov, V.V.¹  Toropova, F.V.¹  Vereninov, A.A.¹ 

[1] Institute of Cytology, Academy of Sciences, St. Petersburg, Russian Federation

关键词: Cell proliferation;    Lymphocyte activation;    Human gene expression;    Na+;    K+-ATPase mRNA;    Potassium flux;    Actinomycin D;   

DOI  :  10.1016/0014-5793(93)81732-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Increase in Na⁺,K⁺-ATPase mRNAs was detected in activated lymphocytes by the RT-PCR method. α1 subunit mRNA gradualy increased with time and by 36 h was 2.4 times higher than at the start. Increase in the β1 mRNA was transient reaching a maximum in the 8 h probe and declining to the initial level in the 24 and 36 h probes. The elevation of Na⁺,K⁻-ATPase mRNAs does not underlie a cycloheximide-inhibited increase in cation pumping peculiar to the prereplicative period as can be judged from the fact that Act D fails to eliminate PHA-induced enhancement of pump fluxes.

【 授权许可】

Unknown