期刊论文详细信息
FEBS Letters | |
The effect of Ca2+ on the stability of chicken erythrocyte histone octamers | |
Pretorius, Linda2  Thompson, Patricia1  Purves, Langley R.1  von Holt, Claus1  Lindsey, George G.2  | |
[1] Department of Chemical Pathology, University of Cape Town Republic of South Africa;Chromatin Research Unit, Council for Scientific and Industrial Research, Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch 7700 Republic of South Africa | |
关键词: Histones; Octamer; Reconstitution; Calcium; Erythrocyte chromatin; | |
DOI : 10.1016/0014-5793(82)81116-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Histones can be extracted from chicken erythrocyte chromatin with 2 M CaCl2 10 mM Tris (pH 7.4). The core histones so extracted exist as H3–H4 tetramers and H2A–H2B dimers since calcium at >0.5 M result in dissociation of the histone octamer. Reconstitution of octamers occurs on removal of the Ca2+ by dialysis. Although <0.5 M calcium do not result in octamer dissociation, perturbations in the structure can be detected by CD and tyrosine fluorescence spectroscopy.
【 授权许可】
Unknown
【 预 览 】
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RO201912020283721ZK.pdf | 422KB | download |