The effect of Ca2+ on the stability of chicken erythrocyte histone octamers

FEBS Letters

Pretorius, Linda²  Thompson, Patricia¹  Purves, Langley R.¹  von Holt, Claus¹  Lindsey, George G.² 

[1] Department of Chemical Pathology, University of Cape Town Republic of South Africa;Chromatin Research Unit, Council for Scientific and Industrial Research, Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch 7700 Republic of South Africa

关键词: Histones;    Octamer;    Reconstitution;    Calcium;    Erythrocyte chromatin;   

DOI  :  10.1016/0014-5793(82)81116-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Histones can be extracted from chicken erythrocyte chromatin with 2 M CaCl₂ 10 mM Tris (pH 7.4). The core histones so extracted exist as H3–H4 tetramers and H2A–H2B dimers since calcium at >0.5 M result in dissociation of the histone octamer. Reconstitution of octamers occurs on removal of the Ca²⁺ by dialysis. Although <0.5 M calcium do not result in octamer dissociation, perturbations in the structure can be detected by CD and tyrosine fluorescence spectroscopy.

【 授权许可】

Unknown