Octamer reconstitution from acid‐extracted chicken erythrocyte histones

FEBS Letters

Pretorius, Linda¹  Thompson, Patricia¹  Purves, Langley R.²  von Holt, Claus¹  Lindsey, George G.¹ 

[1] Chromatin Research Unit, Council for Scientific and Industrial Research, Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch 7700, Republic of South Africa;Department of Chemical Pathology, University of Cape Town, Republic of South Africa

关键词: Histone Octamer Reconstitution;   

DOI  :  10.1016/0014-5793(82)80625-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Histone octamers have been reconstituted from acid-extracted chicken erythrocyte histones. By the criteria of molecular size on exclusion chromatography as well as sedimentation velocity and conformational properties established by circular dichroism, fluorescence spectroscopy and imido-ester cross-linking, the reconstituted octamers have a structure identical to that of salt-extracted octamers.

【 授权许可】

Unknown