Highly conserved hexamer, octamer and nonamer motifs are positive cis‐regulatory elements of the wheat histone H3 gene

FEBS Letters

Fujimoto, Yoshinobu¹  Yang, Ping¹  Ito, Takuya¹  Minami, Maki¹  Nakayama, Takuya¹  Iwabuchi, Masaki¹  Sakamoto, Ayako¹ 

[1] Department of Botany, Faculty of Science, Kyoto University, Kyoto 606-01, Japan

关键词: Transcription;    Wheat histone H3 gene;    Hexamer;    Octamer;    Nonamer;    HBP-1 family;    HBP;    histone gene-binding protein;    GUS;    β-glucuronidase;    NOS;    nopaline synthase;   

DOI  :  10.1016/0014-5793(92)80188-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Base substitution mutations were introduced into the promoter region of the wheat histone H3 gene and promoter activity was assayed in stably transformed sunflower calli or in wheat protoplasts transfected transiently. At least four positive regulatory elements, a hexamer motif (ACGTCA). two octamer(-like) motifs of a direct (CcCGGATC) and a reverse (aATCCGCG) form, and a nonamer motif (CATCCAACG) were identified within the -185 region of the H3 promoter. Analyses of the type I element (CCCC) consisting of the hexamer and reverse-oriented octamer motifs, and which is conserved in other plant histone genes as well, predicted the presence of an octamer-binding protein(s).

【 授权许可】

Unknown