Rapid, transient methylation of four proteins in aggregative amoebae of Dictyostelium discoideum as a response to stimulation with cyclic AMP

FEBS Letters

van Waarde, Aren¹ 

[1] Cell Biology and Morphogenesis Unit, Zoological Laboratory, University of Leiden, Kaiserstraat 63, 2311 GP Leiden, The Netherlands

关键词: Protein carboxymethylation;    Chemotaxis;    Cyclic AMP;    Dictyostelium discoideum;    Signal transduction;    Cell aggregation;   

DOI  :  10.1016/0014-5793(82)81114-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Dictyostelium discoideum, extracellular cAMP induces chemotaxis and cell aggregation. Suspensions of cAMP-sensitive cells are shown to respond to a 10⁻⁶M cAMP-pulse with increased methylation of 4 proteins with app. M _r 110000, 46000, 28000 and 16000. The M _r 110000 and 28000 proteins show a triphasic response with maxima 15, 60 and 150–180s after stimulation. The responses of the M _r 46000 and 16000 proteins are monophasic, maxima being reached 3 and 15s after stimulation, respectively. Optimal responses of methylation are observed over 10⁻⁷−10⁻⁶M cAMP. The methylation reaction may be involved in the processing of the chemotactic signal.

【 授权许可】

Unknown