Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA

FEBS Letters

Tsuji, Frederick I.³  Takeuchi, Ikuo¹  Iida, Hidetoshi¹  Nakao, Hajime¹  Saran, Shweta¹  Tasaka, Masao¹  Nanjundiah, Vidyanand² 

[1] National Institute for Basic Biology, 38 Nishigonaka, Myodaiji Okazaki 444, Japan;Developmental Biology and Genetics Laboratory, Indian Institute of Science, Bangalore 560 012, India;Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565, Japan

关键词: Dictyostelium discoideum;    Aequorin;    Cyclic AMP;    Calcium;    Cell differentiation;   

DOI  :  10.1016/0014-5793(94)80626-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new method is described for measuring intracellular free calcium concentrations, [(Ca²⁺ _i)], in the cells of Dictyostelium discoideum transformed with apoaequorin cDNA of the jellyfish, Aequorea victoria. Aequorin, a calcium-specific indicator, was regenerated in vivo from apoaequorin produced in the cells by incubation with coelenterazine. The results showed that [(Ca²⁺)_i] in developing cells markedly increases at the aggregation stage and again at the culmination stage after a temporary drop at the migration stage. Except for the vegetative stage, the cells at all stages of development exhibit a sharp transient increase in [(Ca²⁺)_i] upon stimulation with a cAMP (50 nM) pulse, high responses being observed at the migration and culmination stages. Separated prestalk cells of migrating slugs contain more than twice as much [(Ca²⁺)_i] and show three times as large a response to cAMP stimulation as prespore cells.

【 授权许可】

Unknown