Chemoattractant‐induced membrane hyperpolarization in Dictyostelium discoideum A possible role for cyclic GMP

FEBS Letters

Wang, Mei¹  Van Duijn, Bert¹ 

[1] Cell Biology and Genetics Unit, Zoological Laboratory, Leiden University, Kalserstraat 63, NL-2311 GP Leiden, The Netherlands

关键词: K+ channel;    H+ −ATPase;    Cyclic AMP;    Chemotaxis;    Cyclic GMP;    Dictyostelium discoideum;    TPP+;    tetraphenylphosphonium cation;    DES;    diethylstilbestrol;    E m;    membrane potential;    E hyp;    membrane potential at 2 min after cAMP stimulation;   

DOI  :  10.1016/0014-5793(90)81471-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membrane potentials of the cellular slime mold Dictyostelium discoideum were monitored after chemotactic stimulation by measuring the distribution of the lipophilic cation tetraphenylphosphonium. Stimulation with the chemoattractant cAMP induces a transient membrane hyperpolarization which reaches its most negative value between 1 3 min after stimulation. This hyperpolarization is consistent with the opening of potassium channels. Measurements in streamer F mutant cells reveal that cGMP likely plays a role in the regulation of the cAMP-induced hyperpolarization.

【 授权许可】

Unknown