Synthesis of rat muscle carbonic anhydrase III in a cell‐free translation system

FEBS Letters

Shephard, Elizabeth¹  Phillips, Ian¹  Carter, Nicholas²  Shiels, Alan²  Jeffrey, Stephen² 

[1] Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, England;Department of Child Health, St. George's Hospital Medical School, London SW17 0RE England

关键词: Carbonic anhydrase;    Immunoprecipitation;    mRNA;    Radioimmunoassay;    Muscle;    Translation in vitro;   

DOI  :  10.1016/0014-5793(82)81256-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS—polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same M _r-value as the native isoenzyme. CA III mRNA was found to represent ∼0.55% of the total mRNA present in rat soleus muscle.

【 授权许可】

Unknown