Synthesis of carbonic anhydrase with messenger RNA isolated from the cells of Chlamydomonas reinhardtii Dangeard C‐9 grown in high and low CO2

FEBS Letters

Toguri, T.¹  Okabe, K.¹  Yang, S.Y.¹  Miyachi, S.¹ 

[1] Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Carbonic anhydrase;    Cell-free translation;    Immunoprecipitation;    Chlamydomonas reinhardtii;    mRNA;    CA;    carbonic anhydrase;    high-CO2 cells;    algal cells grown in air containing 4% CO2;    low-CO2 cells;    algal cells grown in ordinary air (containing 0.04% CO2);    PAGE;    polyacrylamide gel electrophoresis;    pcv;    packed cell volume;   

DOI  :  10.1016/0014-5793(84)81381-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Poly(A)⁺RNA was isolated from Chlamydomonas reinhardtii cells grown autotrophically in ordinary air (low-CO₂ cells) and in air enriched with 4% CO₂ (high-CO₂ cells). In vitro translation products with the messenger RNA showed that the synthesis of the 37-kDa peptide was greater in low-CO₂ than in high-CO₂ cells. The immunoprecipitate produced by treating the translation products with antiserum against carbonic anhydrase (CA) from this alga was also 37 kDa. Comparison of radioactivities incorporated into these 37 kDA peptides suggested that CA induction by lowering atmospheric CO₂ concentration is regulated at the transcriptional level.

【 授权许可】

Unknown