FEBS Letters | |
Enhancing effect of the 3′‐untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro | |
Spirin, Alexander S.1  Zeyenko, Vladimir V.1  Gallie, Daniel R.2  Ryabova, Lyubov A.1  | |
[1] Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow region, Russian Federation;Department of Biochemistry, University of California, Riverside, CA 92521-0129, USA | |
关键词: Viral 3′-untranslated region; Recombinant mRNA; Cell-free translation; mRNA stability; | |
DOI : 10.1016/0014-5793(94)01126-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In order to test the enhancing effect of the 3′-terminal untranslated region (3′-UTR) of tobacco mosaic virus (TMV) RNA on protein synthesis in vitro we used a chimeric mRNA construct containing TMV 5′-UTR (Ω) and firefly luciferase mRNA. The addition of the TMV 3′-UTR to the chimeric mRNA construct results in a more than 3-fold stimulation of the synthesis of the functionally active protein in the wheat germ cell-free translation system. We have demonstrated that the proper length of the TMV 3′-terminal part is important for efficient translation; elongation of the TMV tail by 160 vector-derived nucleotides fully abolishes the stimulation effect of the TMV 3′-UTR in vitro.
【 授权许可】
Unknown
【 预 览 】
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