Enhancing effect of the 3′‐untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro

FEBS Letters

Spirin, Alexander S.¹  Zeyenko, Vladimir V.¹  Gallie, Daniel R.²  Ryabova, Lyubov A.¹ 

[1] Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow region, Russian Federation;Department of Biochemistry, University of California, Riverside, CA 92521-0129, USA

关键词: Viral 3′-untranslated region;    Recombinant mRNA;    Cell-free translation;    mRNA stability;   

DOI  :  10.1016/0014-5793(94)01126-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to test the enhancing effect of the 3′-terminal untranslated region (3′-UTR) of tobacco mosaic virus (TMV) RNA on protein synthesis in vitro we used a chimeric mRNA construct containing TMV 5′-UTR (Ω) and firefly luciferase mRNA. The addition of the TMV 3′-UTR to the chimeric mRNA construct results in a more than 3-fold stimulation of the synthesis of the functionally active protein in the wheat germ cell-free translation system. We have demonstrated that the proper length of the TMV 3′-terminal part is important for efficient translation; elongation of the TMV tail by 160 vector-derived nucleotides fully abolishes the stimulation effect of the TMV 3′-UTR in vitro.

【 授权许可】

Unknown