Viral Qβ RNA as a high expression vector for mRNA translation in a cell‐free system

FEBS Letters

Spirin, Alexander S.¹  Katanaev, Vladimir L.¹  Kurnasov, Oleg V.¹ 

[1] Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russian Federation

关键词: Qβ phage RNA;    Recombinant mRNA;    Coat protein gene;    Cell-free translation;    Dihydrofolate reductase gene;   

DOI  :  10.1016/0014-5793(95)00006-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dihydrofolate reductase (DHFR) mRNA was inserted into Qβ phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Qβ replicase as compared with its synthesis when the original Qβ RNA was used.

【 授权许可】

Unknown