Expression and stability of recombinant RQ‐mRNAs in cell‐free translation systems

FEBS Letters

Spirin, Alexander S.¹  Chetverin, Alexander B.¹  Ugarov, Victor I.¹  Jung, Gyoo Y.¹  Morozov, Igor Yu.¹ 

[1] Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russian Federation

关键词: Qβ phage;    Satellite RQ RNA;    Recombinant mRNA;    Cell-free translation;    mRNA stability;    DHFR;    dihydrofolate reductase;    CAT;    chloramphenicol acetyl transferase;   

DOI  :  10.1016/0014-5793(94)80255-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Qβ. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.

【 授权许可】

Unknown