HeLa cell messenger RNAs of widely differing sizes encode certain heat shock proteins in the M r region of 72 000–74 000

FEBS Letters

Sillar, Gillian M.¹  Cato, Andrew C.B.¹  Kioussis, Joanna¹  Burdon, Roy H.¹ 

[1] Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland

关键词: HeLa cell;    Heat shock response;    Heat shock protein;    Messenger RNA;    Pre-messenger RNA;   

DOI  :  10.1016/0014-5793(82)81206-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cloned cDNA sequences coding for specific 72 000–74 000 M _r HeLa cell heat shock proteins (HSPs) have been used as probes to examine cytoplasmic and nuclear RNA of heat shocked HeLa cells. The mRNAs for these HSPs vary widely in size. Whilst the β-, δ- and ε-HSPs are encoded in mRNAs of 1.9 kb, the γ-HSP arises from an mRNA three times that size. A search for putative nuclear precursors to these mRNAs revealed high M _r candidates at 15.8 kb which accumulate 1–2 h after the initial heat shock, thus supporting the notion that transcriptional control is important in the initial heat shock response.

【 授权许可】

Unknown