An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG

Bulletin of the Korean chemical society

Ji Hye Choi¹  Seung Cheol Chang¹  Seung Jae Yi¹  Deog Su Park¹  Hwa Jung Kim¹  Chulhun L. Chang¹  Kyung Chun Kim¹ 

关键词: Immunochip;    Enzyme immunoassay;    Capacitance;    Impedometric;    Interdigitated electrode;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

This study describes the development of an electrochemical array immunochip for the detection of IgG. Interdigitated immunochip platforms were fabricated by sputtering gold on a glass wafer by using MEMS process and then were coated with Eudragit S100, an enteric polymer, forming an insulating layer over the working area of immunochips. The breakdown of the polymer layer was exemplified by the catalytic action of urease which, in the presence of urea, caused an alkaline pH change. This subsequently caused an increase of the double layer capacitance of the underlying electrode. Used in conjunction with a competitive immunoassay format, this allowed the ratio of initial to final electrode capacitance to be directly linked with the concentration of analyte, i.e. IgG. Responses to IgG could be detected at IgG concentration as low as 250 ngmL−1 and showed good linearity up to IgG concentration as high as 20 μgmL−1.

【 授权许可】

Unknown