Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

Revista da Sociedade Brasileira de Medicina Tropical

Claudia Lamarca Vitral¹  Ana Maria Coimbra Gaspar¹  Clara Fumiko Tachibana Yoshida¹ 

关键词: Enzyme immunoassay;    Anti-HAV;    Hepatitis A;    Enzyme immmunoassay;    Anti-HAV;    Hepatitis A;   

DOI  :  10.1590/S0037-86821991000200003

来源: SciELO

【 摘 要 】

Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License