Development of an enzyme immunoassay for poliovirus antigens

Brazilian Archives of Biology and Technology

Newton Hashimoto¹  Fabrício José Benati¹  Flávio Lauretti¹  Rosa Elisa Carvalho Linhares¹  Carlos Mitihiko Nozawa¹ 

关键词: Enzyme immunoassay;    Poliovirus antigen;   

DOI  :  10.1590/S1516-89132007000100005

来源: SciELO

【 摘 要 】

An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.

【 授权许可】

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