Quality Evaluation of Moutan Cortex Radicis Using Multiple Component Analysis by High Performance Liquid Chromatography

Bulletin of the Korean chemical society

KiHwan Bae¹  Young Ho Kim¹  Kyung Lae Park¹  Yan Ding¹  Enqi Wu¹  Jong Seong Kang¹  Jianbo Chen¹  Thi Ha Do¹  Huu Tung Nguyen¹ 

关键词: Moutan Cortex Radicis;    HPLC;    Qualitative evaluation;    Chromatographic fingerprint;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

A simple high performance liquid chromatographic method was developed to evaluate the quality of Moutan Cortex Radicis based on chromatographic fingerprints that characterize eight pharmacological compounds, namely, gallic acid, paeoniflorin, galloyl paeoniflorin, benzoic acid, quercetin, benzoylpaeoniflorin, paeoniflorigenone, and paeonol. These compounds were identified by their characteristic UV profiles and the mass spectroscopy data, and their contents were determined by HPLC. The chromatographic separation was performed on a C₁₈ column by gradient elution with 0.05% formic acid in water and acetonitrile. The methodological validation gave acceptable linearities (r = 0.9996) and recoveries (ranging from 99.4�?103.1%). The limits of detection (LOD) of these compounds ranged from 10 to 30 レg/mL. The representative chromatographic fingerprints of Moutan Cortex Radicis were obtained by analyzing 20 batches of samples collected from markets in Korea and China. For the efficient evaluation of quality for the commercial Moutan Cortex Radicis it is recommended that the total content of the six characteristic compounds should contain more than a minimum of 2% and that the content of total paeoniflorin and paeonol should exceed a minimum of 1.5% of dry weight of Moutan Cortex Radicis.

【 授权许可】

Unknown