Discrimination of BaphicacanthisCusiae Rhizoma et radix and its adulterant species and establishment of an assay method for quality control

Chinese Medicine

Research

Guo-Kai Huang¹  Xiao-Xiao Liu¹  Yue Li²  Zhi-Hong Jiang²  Lee-Fong Yau²  Qiong-Xi Yu²  Jing-Guang Lu²  Jing-Rong Wang³ 

[1] Guangdong Institute for Drug Control, 510663, Guangzhou, China;State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, 999078, Taipa, Macao, China;State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, 999078, Taipa, Macao, China;State Key Laboratory of Dampness, Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510000, Guangzhou, China;Guangdong-Hong Kong-Macau Joint Lab On Chinese Medicine and Immune Disease Research, 510000, Guangzhou, China;

关键词: UHPLC Q-TOF-MS;    Chromatographic fingerprint;    Quantitative markers;    Isatis indigotica;    2-Benzoxazolinone;    Acteoside;   

DOI  :  10.1186/s13020-023-00777-x

 received in 2023-03-24, accepted in 2023-05-26,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundBaphicacanthisCusiae Rhizoma et Radix, commonly known as Nan-Ban-Lan-Gen (NBLG), is an essential traditional Chinese medicine that possesses diverse bioactivities, particularly noteworthy for its antiviral properties. Although NBLG has been listed in the Chinese Pharmacopoeia as an independent Chinese medicine, the establishment of a comprehensive quality standard for NBLG remains elusive. The absence of assay for marker compound in its quality standards has led to the lack of corresponding quality control measures for NBLG-containing preparations, and its discrimination from adulterant species in the market which thereby can significantly impact the efficacy and safety of NBLG-containing products.MethodsUltra-high performance liquid chromatography (UHPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) was employed for comprehensive profiling of the chemical constituents of NBLG, the stem of Baphicacanthus cusia (Nees) Bremek (NBLJ), and the roots of Isatis indigotica Fort. (Bei-Ban-Lan-Gen, BBLG). Additionally, multivariate analysis was conducted to compare the chemical components of NBLG with those of NBLJ and BBLG. Furthermore, we established an optimized and validated HPLC method to obtain the fingerprint of NBLG and quantify the content of 2-benzoxazolinone and acteoside in the samples.ResultsA total of 73 compounds belonging to six classes were assigned in NBLG, with alkaloids being the most abundant and diverse species. High compositional similarities with significant differences in content were observed between NBLG and NBLJ. Moreover, the chemical profile of BBLG markedly differed from that of NBLG. An informative high performance liquid chromatography (HPLC) fingerprint of NBLG comprising seven characteristic peaks that can be used for quality assessment was established. Notably, we propose a quality control standard for NBLG, stipulating that the limit of content in dry weight for both 2-benzoxazolinone and acteoside should not be less than 0.010%.ConclusionThis study provides the most comprehensive chemical information to date on NBLG, offering valuable insights into its authentication and quality control. Our findings highlight the importance of comprehensive chemical profiling to differentiate potential substitutions and adulterations of herbal medicines, particularly when the original source is scarce or unavailable. These results can aid in the development of quality control measures for NBLG-containing preparations, ensuring their safety and efficacy.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

40517_2023_252_Article_IEq37.gif

40517_2023_252_Article_IEq31.gif

Fig. 4

Fig. 4

Fig. 3

Fig. 2

42004_2023_911_Article_IEq36.gif