Bulletin of the Korean chemical society | |
Electrochemical and Spectroscopic Studies on the Interaction between DNA and the Product of Enzyme-catalyzed Reaction of OPD-H2O2-HRP | |
L. B. Ma1  S. Y. Niu1  K. Jiao1  S. S. Zhang1  | |
关键词: 2; 3-Diaminophenazine; DNA; Cyclic voltammetry; UV/Vis spectroscopy; Intercalation; | |
DOI : | |
学科分类:化学(综合) | |
来源: Korean Chemical Society | |
【 摘 要 】
The pure product of 2,3-diaminophenazine was prepared by the enzyme-catalyzed reaction of ophenylenediamine-H2O2-horseradish peroxidase and characterized by UV/Vis spectroscopy, IR spectroscopy and NMR spectroscopy. The electrochemical behaviour of 2,3-diaminophenazine on the glassy carbon electrode was studied. The interaction between 2,3-diaminophenazine and deoxyribonucleic acid was studied by cyclic voltammetry method and UV/Vis spectroscopy, which indicated that the interaction between them is intercalation. The influence of reacting time was also studied. The binding ratio of the 2,3-diaminophenazine-DNA complex is calculated to be 1 : 2 and the binding constant is to be 5.07 �? 103 L·mol?1 at room temperature.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912010239388ZK.pdf | 143KB | download |