Soft Material Programming through the Spatiotemporal Release of Oligonucleotides

DNA;Spatiotemporal;4D Printing;Chemical & Biomolecular Engineering

Rubanov, MosheSchulman, Rebecca B ;

Johns Hopkins University

关键词: DNA;    Spatiotemporal;    4D Printing;    Chemical & Biomolecular Engineering;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/60174/RUBANOV-THESIS-2018.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

The programmable spatiotemporal release of molecular outputs is a prerequisite for creating new classes of stimuli responsive biomaterials capable of executing sensing and computational programs. In this thesis, we demonstrate the ability to fabricate heterogeneous micromaterials that implement sequentially activated molecular cascades with programmed timescales of activation and release from a hydrogel. These materials use DNA-based strand-displacement to direct the sequential release of short oligonucleotides from spatial domains. Digital maskless photolithography enables the spatial sequestration of acrylate-modified short oligonucleotides for local hybridization reactions within hydrogels at sizes of tens of microns. To control temporal release, we used a toehold mediated DNA strand displacement reaction cascade that enables the controlled release of oligonucleotides at 8-hour intervals. This system could be used to direct chemo-mechanical actuation within soft-robots or as a local chemical clock to sequentially coordinate movement. The programmed release of DNA oligonucleotides from hydrogel substrates enables the scalable development of DNA-based reaction-diffusion systems that regulate the availability of oligonucleotides at different points in space and time.