SPECIFIC DNA SEQUENCE RESPONISIVE DNA CROSSLINKED HYDROGEL AND ITS APPLICATIONS

DNA;Hydrogel;Chemical & Biomolecular Engineering

Huang, Qi

Johns Hopkins University

关键词: DNA;    Hydrogel;    Chemical & Biomolecular Engineering;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/44640/HUANG-THESIS-2017.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

In the first theme of the thesis, we show hydrogel architectures photopatternedby DNA co-polymer that can be actuated by up to three different DNA sequence. Thehydrogel architectures show great shape change accurately at the region we design afterthe specific biomolecular signal is applied to the system. This combination ofmicrofabrication technology with DNA nanotechnology allow us to build tiny softdevices or soft robots that can be actuated by particular DNA sequence.The second theme of this thesis is generating DNA crosslinked microgelthrough microfluidic methods. To apply the method, we first form emulsion dropletsiiiout of microfluidic device then solidify the droplets by subsequent UV triggeredpolymerization. In this droplets generating process, by tuning the flow rate of thecontinuous phase and the dispersed phase, microfluidic generation of droplets can bewell controlled over size and monodispersity. With this strategy, we can generate agreat amount of microgels with uniform spherical geometry in a relatively simplerprocess and a faster speed than microfabrication and template particle synthesis.