Characterization and determination of the redox properties of the 2[4Fe‐4S] ferredoxin from Methanosarcina barkeri strain MS

FEBS Letters

Vogels, Godfried D.²  Daas, Piet J.H.²  Hagen, Wilfred R.¹  Keltjens, Jan T.² 

[1] Department of Biochemistry, Agricultural University, Wageningen, The Netherlands;Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525 ED, Nijmegen, The Netherlands

关键词: Ferredoxin;    Iron-sulfur cluster;    Cyclic voltammetry;    Redox potential;    Methanosarcina barkeri;   

DOI  :  10.1016/0014-5793(94)01313-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ferredoxin was purified from methanol-grown Methanosarcina barkeri strain MS. It was isolated as a dimer with a subunit molecular weight of 6,200. The protein contained 7.4 mol iron and 7.2 mol acid-labile sulfur per monomer. In the reduced state the ferredoxin exhibited an EPR spectrum characteristic of two spin-coupled [4Fe-4S]¹⁺ clusters. The E _m of the [4Fe-4S]^2+;1+ couple was −322 mV ± 3 mV vs. NHE at 21°C and pH 7.0. The midpoint potential was temperature but not pH dependent. At the physiological temperature of 37°C the E _m was −340 mV.

【 授权许可】

Unknown