microPET-Based Biodistribution of Quantum Dots in Living Mice

Journal of Nuclear Medicine

Gopal Iyer¹  Jianghong Rao¹  Anna M. Wu¹  Sheen-Woo Lee¹  Laurent A. Bentolila¹  Meike L. Schipper¹  Shimon Weiss¹  Xiaoyuan Chen¹  Sanjiv S. Gambhir¹  Zhen Cheng¹ 

关键词: quantum dot;    biodistribution;    nanoparticle;    PET;    molecular imaging;   

DOI  :  10.2967/jnumed.107.040071

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice. Methods: 64Cu-Labeled 800- or 525-nm emission wavelength QD (21- or 12-nm diameter), with or without 2,000 MW (molecular weight) polyethylene glycol (PEG), were injected intravenously into mice (5.55 MBq/25 pmol QD) and studied using well counting or by serial microPET and region-of-interest analysis. Results: Both methods show rapid uptake by the liver (27.4–38.9 %ID/g) (%ID/g is percentage injected dose per gram tissue) and spleen (8.0–12.4 %ID/g). Size has no influence on biodistribution within the range tested here. Pegylated QD have slightly slower uptake into liver and spleen (6 vs. 2 min) and show additional low-level bone uptake (6.5–6.9 %ID/g). No evidence of clearance from these organs was observed. Conclusion: Rapid reticuloendothelial system clearance of QD will require modification of QD for optimal utility in imaging living subjects. Formal quantitative biodistribution/imaging studies will be helpful in studying many types of nanoparticles, including quantum dots.

【 授权许可】

Unknown