Elevated O-GlcNAcylation of Extracellular Vesicle Proteins Derived from Metastatic Colorectal Cancer Cells

Cancer Genomics - Proteomics

JISNUSON SVASTI²  CHANTRAGAN SRISOMSAP²  PARUNYA CHAIYAWAT³  PUKKAVADEE NETSIRISAWAN¹  CHURAT WEERAPHAN¹  VORARATT CHAMPATTANACHAI²  DARANEE CHOKCHAICHAMNANKIT¹ 

[1] aboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand;pplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailandpplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailandpplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailandpplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailandaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand;pplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailandpplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailandpplied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailand

关键词: Colorectal cancer;    extracellular vesicles;    O-GlcNAcylation;    metastasis;    RuVB-like1;    transitional endoplasmic reticulum ATPase;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: O-GlcNAcylation is a single sugar attachment of serine and/or threonine residues on intracellular proteins. Recent reports reveal that it can modify several secretory proteins; however, the underlying mechanisms are largely unexplored. Materials and Methods: To investigate whether extracellular vesicles (EVs) carry secretory O-GlcNAc-modified proteins that were isolated from colorectal cancer (CRC) cells, two-dimensional gel electrophoresis followed with O-GlcNAc immunoblotting and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied. Results: It was revealed that the O-GlcNAc modification of many EV proteins was increased in metastatic cells. Among these, transitional endoplasmic reticulum ATPase (TER ATPase) and RuVB-like1 were successfully confirmed for the O-GlcNAc modification in which the levels were significantly higher in EVs of metastatic CRC cell line. Conclusion: These data, demonstrate that proteins carried by EVs are O-GlcNAc-modified. Importantly, elevated aberrant O-GlcNAcylation of EV proteins might serve as a potential biomarker of metastatic CRC.

【 授权许可】

Unknown