Selectively-Packaged Proteins in Breast Cancer Extracellular Vesicles Involved in Metastasis

International Journal of Molecular Sciences

BruceK. Milthorpe¹  Jerran Santos¹  MatthewP. Padula²  PenelopeV. Dalla³ 

[1] Advanced Tissue Engineering and Stem Cell Biology Group, School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney NSW 2007, Australia;Proteomics Core Facility, School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney NSW 2007, Australia;Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, The University of Sydney, Sydney NSW 2037, Australia;

关键词: extracellular vesicles;    metastasis;    breast cancer;    cytokines;    proteomics;   

DOI  :  10.3390/ijms21144990

来源: DOAJ

【 摘 要 】

Cancer-derived extracellular vesicles are known to play a role in the progression of the disease. In this rapidly-growing field, there are many reports of phenotypic changes in cells following exposure to cancer-derived extracellular vesicles. This study examines the protein contents of vesicles derived from three well-known breast cancer cell lines, MCF-7, MDA-MB-231 and T47D, using peptide-centric LC-MS/MS and cytokine multiplex immunoassay analysis to understand the molecular basis of these changes. Through these techniques a large number of proteins within these vesicles were identified. A large proportion of these proteins are known to be important in cancer formation and progression and associated with cancer signaling, angiogenesis, metastasis and invasion and immune regulation. This highlights the importance of extracellular vesicles (EVs) in cancer communications and shows some of the mechanisms the vesicles use to assist in cancer progression.

【 授权许可】

Unknown