Phospho-Network Analysis Identifies and Quantifies Hepatitis C Virus (HCV)-induced Hepatocellular Carcinoma (HCC) Proteins Regulating Viral-mediated Tumor Growth

Cancer Genomics - Proteomics

SAMUEL W. FRENCH⁵  JAMES Q. VU⁵  JOE CAPRI⁴  MARY ZIEGLER⁵  JULIAN WHITELEGGE³  NIKITA PATEL⁵  ANGELA TRAMONTANO⁶  NATALIE M. LIU⁵  NU T. LU²  WHITAKER COHN³  ROGER WILLIAMS¹  DARSHIL PATEL⁵ 

[1] he Foundation for Liver Research-The Institute for Hepatology, London, U.K.he Foundation for Liver Research-The Institute for Hepatology, London, U.K.he Foundation for Liver Research-The Institute for Hepatology, London, U.K.;epartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Hematology and Oncology, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Hematology and Oncology, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Hematology and Oncology, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Hematology and Oncology, University of California Los Angeles, Los Angeles, CA, U.S.A;epartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.A;epartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles CA, U.S.Aepartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles CA, U.S.Aepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles CA, U.S.Aepartment of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles CA, U.S.A;epartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.Aepartment of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.A;nstitute for Technology Assessment, Massachusetts General Hospital, Boston, MA, U.S.Anstitute for Technology Assessment, Massachusetts General Hospital, Boston, MA, U.S.Anstitute for Technology Assessment, Massachusetts General Hospital, Boston, MA, U.S.A

关键词: Hepatocellular carcinoma;    hepatitis C virus;    mass spectrometry;    phosphoproteomics;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Patients with chronic hepatitis C virus (HCV) infection are at risk of serious complications of cirrhosis and hepatocellular carcinoma (HCC). Mass spectrometry (MS) is a versatile methodology that produces a global proteomic landscape for analysis of cancer mechanisms. Materials and Methods: Using multiplex peptide stable isotopic labeling and immobilized metal affinity chromatography (IMAC), we enriched and quantified the phosphoproteome of HCC, with and without HCV. While raw data identified protein targets based on expression alone, we also used abundance groups for comprehensive functional analysis. Results: Analysis of functional differences highlighted deregulated phosphoprotein networks. This uncovered additional candidates that could be directly derived from the MS data. Cellular processes and pathways that may differ with HCV infection include: cytoskeletal dynamics, insulin response, gene expression, and PI3K/AKT oncogenesis. Conclusion: This function-focused workflow provides a simple framework to analyze MS data. Phosphoproteome quantitation with inclusive functional analysis can generate hypotheses for liver cancer research to improve early screening and identification of molecular targets for therapy.

【 授权许可】

Unknown