Investigation of curcumin-mediated signalling pathways in head and neck squamous cell carcinoma:

Translational Research in Oral Oncology

VishalakshiNanjappa¹ 

关键词: Kinases;    phosphoproteomics;    signalling pathways;    mass spectrometry;    quantitative proteomics;    bioavailability;    head;    neck cancer;    calcium/calmodulin-dependent protein kinases;   

DOI  :  10.1177/2057178X17743142

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Objectives:Curcumin has been shown to exhibit anti-neoplastic effects. However, due to its poor bioavailability, the use of curcumin as an anti-cancer drug is limited. Thus, it is necessary to identify molecules as an alternative to curcumin that could serve as anti-cancer targets. In this study, we attempted to understand the underlying curcumin-mediated signalling pathways contributing to anti-neoplastic effects of curcumin.Methods:We carried out mass spectrometry-based phosphoproteomic analysis of head and neck cancer cell line, CAL 27, treated with and without curcumin to identify curcumin-mediated signalling pathways. Serine/threonine kinases were enriched using titanium dioxide.Results:This resulted in the identification of 5921 phosphopeptides corresponding to 1878 proteins. Of these, 275 and 183 phosphopeptides corresponding to 335 and 242 proteins (≥2.0-fold) were found to be hyper- and hypo-phosphorylated, respectively, in response to curcumin treatment. Calcium/calmodulin-dependent protein kina...

【 授权许可】

CC BY