Activation of EphA receptors on CD4+CD45RO+ memory cells stimulates migration

Journal of Leukocyte Biology

K. Nustad²  H.-C. Aasheim¹  H. L. Holen¹ 

[1] Department of Medical Genetics, Ullevaal University Hospital, Oslo, Norway; and Department of Medical Genetics, Ullevaal University Hospital, Oslo, Norway; and Department of Medical Genetics, Ullevaal University Hospital, Oslo, Norway; and;Department of Medical Biochemistry, Radiumhospitalet, Rikshospitalet Medical Centre, Oslo, Norway Department of Medical Biochemistry, Radiumhospitalet, Rikshospitalet Medical Centre, Oslo, Norway Department of Medical Biochemistry, Radiumhospitalet, Rikshospitalet Medical Centre, Oslo, Norway

关键词: tyrosine kinases;    signaling complex;    CXCL12;    ephrin;   

DOI  :  10.1189/jlb.0709497

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

We have demonstrated previously that binding of ephrin-A1 to EphA receptors on human CD4+ and CD8+ T cells stimulates migration. Two EphA receptors have been reported in T cells: EphA1 at the protein level and EphA4 at the mRNA level. In this study, we wanted to investigate the expression profile of these receptors in T cell subpopulations and to test if expression differences would affect the potential of cells to migrate upon ephrin-A1 binding. We have generated an anti-EphA4 mAb for expression analysis. Our data show that functional EphA4 is expressed on the cell surface of CD4+ and CD8+ T cells. In addition, EphA4 receptor expression is induced after overnight incubation in serum-free medium, in particular, on CD4+CD45RO+ T cells. Migration of CD4+ T cells in response to ephrin-A1 is observed for memory cells (CD45RO+) and much weaker for naïve cells (CD45RA+). A signaling complex associated with the EphA4 receptor has also been isolated and includes EphA1, the Src family kinases Fyn and Lck, Slp76, and Vav1. To conclude, T cells express EphA1 and EphA4 receptors. Expression differences of EphA4 are observed in subpopulations of CD4+ T cells. This is related to the cell migration potential after ephrin-A1 binding.

【 授权许可】

Unknown