Activation of EphA receptors on CD4+CD45RO+ memory cells stimulates migration

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: tyrosine kinases;    signaling complex;    CXCL12;    ephrin;   

DOI  :  10.1189/jlb.0709497

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Wehavedemonstratedpreviouslythatbindingofephrin‐A1toEphAreceptorsonhumanCD4+andCD8+Tcellsstimulatesmigration.TwoEphAreceptorshavebeenreportedinTcells:EphA1attheproteinlevelandEphA4atthemRNAlevel.Inthisstudy,wewantedtoinvestigatetheexpressionprofileofthesereceptorsinTcellsubpopulationsandtotestifexpressiondifferenceswouldaffectthepotentialofcellstomigrateuponephrin‐A1binding.Wehavegeneratedananti‐EphA4mAbforexpressionanalysis.OurdatashowthatfunctionalEphA4isexpressedonthecellsurfaceofCD4+andCD8+Tcells.Inaddition,EphA4receptorexpressionisinducedafterovernightincubationinserum‐freemedium,inparticular,onCD4+CD45RO+Tcells.MigrationofCD4+Tcellsinresponsetoephrin‐A1isobservedformemorycells(CD45RO+)andmuchweakerfornaïvecells(CD45RA+).AsignalingcomplexassociatedwiththeEphA4receptorhasalsobeenisolatedandincludesEphA1,theSrcfamilykinasesFynandLck,Slp76,andVav1.Toconclude,TcellsexpressEphA1andEphA4receptors.ExpressiondifferencesofEphA4areobservedinsubpopulationsofCD4+Tcells.Thisisrelatedtothecellmigrationpotentialafterephrin‐A1binding...

【 授权许可】

CC BY