Production of glucose oxidase and catalase by Aspergillus niger free and immobilized in alginate-polyvinyl alcohol beads

The Journal of General and Applied Microbiology

Cecilia Balvantín-García¹  José L. Martínez-Hernández¹  Cristóbal Noé Aguilar¹  Rebeca Betancourt-Galindo²  Elda P. Segura-Ceniceros¹  Anna Ilyina¹  Rosalba De León-Gámez¹  Alma Rosa Paredes Ramírez¹  Rocio V. Garay-Flores¹ 

[1] Coahuila Autonomous University, Nanobioscience Group of Chemistry Faculty and Medical School;Applied Chemistry Research Center

关键词: Aspergillus niger;    catalase;    glucose oxidase;    immobilization in PVA/alginate beads;   

DOI  :  10.2323/jgam.60.262

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The production of glucose oxidase (GOX) and catalase (CAT) by submerged fermentation of the free and immobilized xerophytic fungus Aspergillus niger under equal conditions was compared. To immobilize fungal spores, entrapment in PVA/alginate beads treated with NaNO3/CaCl2 was performed. The yield of immobilization in the beads with a diameter less than 1mm was equal to 100%. Fungus growth and substrate consumption were evaluated in both fermentation systems, demonstrating the lag-period presence in the case of the first cycle of immobilized fungus use. The enzyme production by immobilized fungus reuse was carried out. In these cases, greater enzymatic GOX activity was detected, while CAT activity decreased. SEM micrographs for the beads with immobilized fungus applied in the first and second fermentation cycles were obtained, presenting fungus spreading inside the sphere, spore presence and branching hyphae. Immobilization of A. niger on PVA/alginate beads is effective for GOX and CAT production at least on 2−3 repeated fermentative cycles. Thus, immobilization enables repeated use of microbial cells.

【 授权许可】

Unknown