Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol

Brazilian Archives of Biology and Technology

Jagdish Singh¹  Neelam Verma¹ 

[1] ,Punjabi University Department of Biotechnology Patiala Punjab ,India

关键词: Aspergillus niger;    glucose oxidase;    aqueous two phase;   

DOI  :  10.1590/S1516-89132010000500007

来源: SciELO

【 摘 要 】

The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.

【 授权许可】

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