RAPD分析による栃木県水稲優良品種の品種識別

日本作物学会紀事

吉田 智彦¹  小林 俊一² 

[1] 宇都宮大学農学部;栃木県農業試験場生物工学部

关键词: DNA marker;    DNAマーカー;    Identification of cultivars;    品種識別;    Paddy rice;    水稲;    RAPD analysis;    RAPD分析;    Tochigi pref.;    栃木県;   

DOI  :  10.1626/jcs.74.207

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

The objective of this study was to establish the technology to identify paddy rice cultivars by Ramdom Amplified Polymorphic DNA (RAPD) analysis to raise the quality of rice in Tochigi prefecture and to prevent seed contamination in the foundation seed and the stock seed. A total of 20 paddy rice cultivars including 9 recommended and promising cultivars in Tochigi prefecture, were studied. They could be identified individually by the electrophoresis of the DNA fragments amplified by PCR using 7 random primers, on 1.5% agarose gels and by staining with ethidium bromide.

【 授权许可】

Unknown