RAPD分析による栃木県を中心とした関東周辺地域のムギ類優良品種識別

日本作物学会紀事

吉田 智彦¹  小林 俊一² 

[1] 宇都宮大学農学部;栃木県農業試験場生物工学部

关键词: Barley;    オオムギ;    DNA marker;    DNAマーカー;    Identification of cultivars;    品種識別;    Kanto region;    関東地域;    RAPD analysis;    RAPD分析;    Tochigi pref.;    栃木県;    Wheat;    コムギ;   

DOI  :  10.1626/jcs.75.165

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

The objective of this study was to establish the technology to identify wheat and barley cultivars by random amplified polymorphic DNA(RAPD) analysis to improve the quality of wheat and barley in Tochigi prefecture and to prevent seed contamination in the foundation seed and the stock seed. A total of 17 wheat and 19 barley cultivars in the Kanto region were examined. Wheat cultivars could be identified individually by RAPD analysis using 5 random primers, followed by the electrophoresis of the DNA fragments on 1.5% agarose gels and staining with ethidium bromide. Barley cultivars could be identified individually by RAPD analysis using 6 random primers. A polymorphism was observed among stock seeds collected from various areas in the old wheat cultivar, Norin 61, that was bred in 1945. No polymorphism was observed among F13 pedigrees of two-rowed barley, Kanto Nijo 35, showing that they were fixed.

【 授权许可】

Unknown