国内二条オオムギのDNAマーカーによる品種識別

日本作物学会紀事

吉田 智彦¹  内村 要介²  古庄 雅彦² 

[1] 宇都宮大学;福岡県農業総合試験場

关键词: SSR;    SSR分析;    CAPS;    CAPS分析;    DNA marker;    DNAマーカー;    Barley;    二条オオムギ;    Identification of cultivars;    品種識別;    RAPD;    RAPD分析;   

DOI  :  10.1626/jcs.73.35

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

The objective of this study was to establish the technology to identify barley cultivars by the DNA markers for the purpose of managing the foundation seeds and for protecting the breeder’s right and the brand seeds from pirated cultivars. A total of 24 two–rowed barley cultivars, which consisted of 22 Japanese cultivars and 2 foreign cultivars, were identified individually by cleaved amplified polymorphic sequence (CAPS) analysis of the Restriction Fragment Length Polymorphisms (RFLP) using 9 sequence tagged site (STS) primers and 6 kinds of endonucleases, following the electrophoresis of the DNA fragments in 1.8% agarose gels. In addition, we selected 28 CAPS markers, 1 simple sequence repeats (SSR) marker and 5 randomly amplified polymorphic DNA (RAPD) markers that could clearly and reliably detect DNA polymorphisms among 24 cultivars.

【 授权许可】

Unknown