Effects of Electro- and Chemical Fusion Treatment on Cell Wall Formation and Cell Division of Plant Protoplasts

Japanese Journal of Crop Science

Eizo MAEDA¹  Takeshi TANIGUCHI¹  Salak PHANSIRI¹  Hiroshi MIYAKE¹ 

关键词: Cell division;    細胞分裂;    Cell wall;    細胞壁;    Fusion;    細胞融合;    Protoplast;    プロトプラスト;   

DOI  :  10.1626/jcs.63.706

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

The effects of electro- and chemical fusion procedures on cell wall formation and cell division of lettuce and soybean protoplasts were studied. Judging from Calcofluor White staining, cell wall formation of both lettuce and soybean protoplasts was stimulated by electro-fusion procedure 3 h after application. On the other hand, the cell wall formation of lettuce protoplasts was not affected by the chemical fusion treatment using polyethylene glycol (PEG). However, cell wall formation was inhibited with a very dilute concentration of PEG when lettuce protoplasts were mixed with PEG for 3 h followed by Calcofluor White staining. The enhancement of cell division of soybean protoplasts was observed by the electro-fusion treatment 3 days of culture. By transmission electron microscopy, it was also confirmed that the cell wall was detected on the surface of the electrofusion-treated protoplasts 1 day after culture. A cell wall was not observed on the surface of nontreated protoplasts. Moreover, the endoplasmic reticulum in the treated soybean protoplasts increased compared with nontreated ones.

【 授权许可】

Unknown