Isolation and Culture of Protoplast from Finger Millet (Eleusine coracana) Callus

Japanese Journal of Crop Science

Takeshi TANIGUCHI¹  Hiroshi MIYAKE¹  Totik Sri MARIANI¹ 

关键词: Cell division;    細胞分裂;    Cell wall;    細胞壁;    Finger millet;    シコクビエ;    Protoplast isolation and culture;    プロトプラストの分離と培養;   

DOI  :  10.1626/jcs.61.668

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were released using the enzyme solution containing 2% Cellulase YC and 0.2% Pectolyase Y-23. About 75% of the protoplasts cultured in the MS liquid medium with 0.4 mgl-1 2, 4-D and 0.1 mgl-1 kinetin supplemented with 5% coconut water were started to show regeneration of the cell wall within 12 h. Cell division was observed within 24 h after culture. The protoplasts that underwent division were about 15%. The sustained cell division could lead to microcolony formation in 4 d after culture. However, further growth did not occur and viability gradually decreased during culture.

【 授权许可】

Unknown