Ultrastructural Analysis of Cell Wall Formation and Cell Division of Soybean Protoplasts

Japanese Journal of Crop Science

Eizo MAEDA¹  Takeshi TANIGUCHI¹  Salak PHANSIRI¹  Hiroshi MIYAKE¹ 

关键词: Cell division;    細胞分裂;    Cell wall formation;    細胞壁形成;    Protoplasts;    ダイズ;    Soybean;    透過型電子顕微鏡;    TEM;    微細構造;    Ultrastructure.;    プロトプラスト.;   

DOI  :  10.1626/jcs.62.429

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Protoplasts of soybean (Glycine max (L.) Merr.) were isolated from suspension cultures and morphological changes during culture were observed using transmission electron microscopy. The regeneration of cell walls on the surface of the protoplasts was accompanied by the release of smooth vesicles from cytoplasm to the outside plasma membrane. The vesicles were presumed to carry some wall materials from cytoplasm for cell wall formation. With the elapse of time, the wall materials were gradually deposited and a complex cell wall layer was formed 2-3 d after culture. The fine structure of cellulose microfibrils was clearly observed about 3 d after culture using a polysaccharide staining procedure. During the cell wall formation, numerous smooth vesicles were always found between the plasma membrane and newly synthesized cell wall. Nuclear division and subsequent cytokinesis were observed about 1 d after culture. From the results obtained by the present and previous researches, it may be concluded that cell wall formation usually starts earlier than cell division and then both reactions proceed concurrently and that smooth vesicles play an important role in cell wall formation.

【 授权许可】

Unknown