Rapid Identification of Mycoplasma pulmonis Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Journal of Veterinary Medical Science

Takashi TAMURA²  Koichi MAKIMURA²  Nobuhito HAYASHIMOTO⁴  Mitsuru MATSUMURA¹  Kazuo GOTO¹  Mikachi YAMAMOTO³  Miwa ASAHARA² 

[1] Department of Clinical Laboratory Medicine, School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan;Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan;Laboratory of Space and Environmental Medicine, Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan;Central Institute for Experimental Animals, ICLAS Monitoring Center, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216–0001, Japan

关键词: MALDI-TOF MS;    mouse;    Mycoplasma pulmonis;    rat;   

DOI  :  10.1292/jvms.12-0029

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(1)Mycoplasma species identification is based on biochemical, immunological, and molecular methods that require several days for accurate identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method for identification of bacteria and has recently been introduced into the clinical microbiology laboratory as a rapid and accurate technique. This method allows a characteristic mass spectral fingerprint to be obtained from whole inactivated mycoplasmal cells. In this study, we evaluated the performance of the MALDI-TOF MS for the identification of Mycoplasma by comparison with standard sequence analysis of 16S rRNA. We developed the first database of MALDI-TOF MS profiles of Mycoplasma species, containing Mycoplasma pulmonis, M. arthritidis, and M. neurolyticum, which are the most common pathogens in mice and/or rats, and species-specific spectra were recorded. Using the database, 6 clinical isolates were identified. Six tracheal swabs from 4 mice and 2 rats were cultured on PPLO agar for 4 to 7 days, and the colonies were directly applied to analyze the protein profiles. Five strains were identified as M. pulmonis, and 1 strain from a mouse was identified as M. neurolyticum (spectral scores were >2.00); the results were consistent with the results of the 16S rRNA gene sequence analysis (homologies>97.0%). These data indicate that MALDI-TOF MS can be used as a clearly rapid, accurate, and cost-effective method for the identification of M. pulmonis isolates, and this system may represent a serious alternative for clinical laboratories to identify Mycoplasma species.

【 授权许可】

Unknown