Improved RNA Extraction Method Using the BioMasher and BioMasher Power-Plus

Journal of Veterinary Medical Science

Masatoshi TANIDA³  Yuko KAKU-USHIKI²  Shinkichi IRIE²  Jun SASAKI²  Shunji HATTORI²  Hitomi FUJISAKI²  Kentaro NAKASHIMA¹  Takuji YAMAMOTO²  Tomomi KIRIYAMA²  Yukio MARUTA³  Shunpei SUGIYAMA³  Kana SUZUKI³  Michi SASAKI² 

[1] Institute of Neuroscience, Tokushima Bunri University, 1314–1 Sido, Sanuki, Kagawa 769–2101, Japan;Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan;Lab Co., Ltd., 2–12 Kita 27 Nishi 6, Kita-ku, Sapporo, Hokkaido 001–0027, Japan

关键词: BioMasher;    BioMasher Power-Plus;    mouse;    rat;    RNA extraction;   

DOI  :  10.1292/jvms.12-0213

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(7)Cited-By(1)The BioMasher is a disposable homogenizer that was developed to homogenize bovine brain tissue for bovine spongiform encephalopathy diagnosis. Capable of preventing the biohazard risk from infectious samples, it also prevents cross-contamination among samples. The BioMasher is thus widely used in biochemical research, especially for RNA extraction. Here, we tested a novel BioMasher application for RNA extraction from animal and plant tissues. We also developed a grinding machine specific for the BioMasher, named the BioMasher Power-Plus. We developed RNA extraction protocols using the BioMasher combined with the BioMasher Power-Plus. We compared RNA extraction efficiency of the BioMasher with that of the FastPrep and the glass homogenizer. Though the RNA extraction efficiency by the BioMasher was nearly equivalent to that of the FastPrep and the glass homogenizer, sample preparation time was shorter for the BioMasher. The utility of RNA extraction by the BioMasher was examined in mouse, rat, and tomato tissue samples. In the rodent tissues, the highest extraction efficiency of total RNA was from liver, with lowest efficiency from fibrous tissues such as muscle. The quality of extracted total RNA was confirmed by agarose gel electrophoresis which produced highly visible clear bands of 18S and 28S rRNAs. Reproducibility among different operators in RNA extraction from tomato roots was improved by using the BioMasher Power-Plus. The BioMasher and BioMasher Power-Plus provide an effective and easy homogenization method for total RNA extraction from some rodent and plant tissues.

【 授权许可】

Unknown