Rapid Apoptotic Changes in the Gastric Glandular Epithelium of Rats Administered Intraperitoneally with Fusarenon-X

Journal of Veterinary Medical Science

Noriaki MIYOSHI¹  Tsutomu SHIMIZU¹  Nobuhiro YASUDA¹  Jinjun LI¹ 

[1] Laboratory of Veterinary Pathology, Faculty of Agriculture, Kagoshima University

关键词: apoptosis;    DNA fragmentation;    fusarenon-X;    rat stomach;    TUNEL;   

DOI  :  10.1292/jvms.59.17

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(2)Fusarenon-X (FX) 1.5 mg/kg was administered intraperitoneally (ip) to 6-week-old male Wistar rats for examination of pathologic effects on the glandular stomach. Rats ip-treated with sterilized physiological saline were used as control. FX-administered rats showed dilatation of the stomach with increased fluid contents after 1-4 hr. Light microscopically, a few apoptotic karyopyknosis were seen in chief cells in the basal region at 1 hr postadministration (PA) and mitotic inhibition was evident after 2 hr PA. Marked apoptosis of nuclear pyknosis and cytoplasmic inclusions in both zymogenic and oxyntic cells developed from basal to middle regions of the gastric mucous membrane at 2-4 hr PA with a peak at 3 hr. Apoptotic changes of differentiating neck cells and surface epithelia were less evident. Electron microscopy revealed that the chief cells were the main target of FX-induced apoptotosis. The parietal cells were secondarily involved because they phagocytosed chief cell-derived apoptotic bodies. In situ detection of DNA breaks by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) reaction revealed the positive nuclei after 1 hr PA, which increased with time and reached a peak at 3 hr PA, in accordance with apoptotic changes in histological study. Agarose gel electrophoresis of DNA isolated from the gastric mucosae of FX-administered rats showed ladder pattern of DNA fragments after 1.5 hr PA with the maximum distinctness at 3 hr PA.

【 授权许可】

Unknown