Chemosensitivity assay in mice prostate tumor: Preliminary report of flow cytometry, DNA fragmentation, ion ratiometric methods of anti-neoplastic drug monitoring

Cancer Cell International

Richard Kline¹  Rakesh Sharma¹ 

[1] Departments of Radiology and Medicine, Columbia University, New York, NY 10032 USA

关键词: DNA fragmentation;    ion ratiometric assay;    flow cytometry;    prostate tumor;    apoptosis;    MRI;   

Others  :  796055
DOI  :  10.1186/1475-2867-4-3

 received in 2003-12-30, accepted in 2004-03-19,  发布年份 2004

【 摘 要 】

Flow cytometry, DNA fragmentation, ion ratiomateric analysis and NMR peaks characterized drug chemosensitivity of antineoplastic drugs. Hypotheses were: 1. The chemosensitive effect of different cancer cell lines is characteristic; 2. DNA fragmentation, ion ratiometric analysis suggest apoptosis status of tumor cells.

Methods

PC-3 cell lines were compared with DU-145, LNCaP cell lines in culture for the [Na]_i and [Ca]_i ion sensing dyes, cell death, NMR peaks and apoptosis staining for chemotherapeutic action of different drugs.

Results

DNA fragmentation, ratiometric ions and fluorescence endlabelling plots were characteristic for cell lines and drug response. ³¹P-²³Na NMR spectra showed characteristic high phospho-choline and sodium peaks.

Conclusion

Flow cytometry, DNA fragmentation, ion ratiometric methods and NMR peaks indicated apoptosis and offered in vivo drug monitoring method.

【 授权许可】

   
2004 Sharma and Kline; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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