Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner

The Journal of Veterinary Medical Science

Emel AKSOY¹ 

[1] Department of Virology, Faculty of Veterinary Medicine, Kirikkale University, 71450 Yahsihan, Kirikkale, Turkey

关键词: apoptosis;    caspase;    DNA fragmentation;    flow cytometry;    Schmallenberg virus;   

DOI  :  10.1292/jvms.18-0582

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Schmallenberg virus (SBV), discovered in 2011 in Germany, is associated with clinical manifestations of fever, diarrhea, reduced milk yield, abortions and congenital malformations in ruminants. Despite many studies performed for SBV, there is no detailed research on in vitro apoptotic effect of SBV. This study is aimed to determine apoptosis pathways and role of pro-apoptotic and anti-apoptotic molecules in Vero cells infected with SBV. The study results showed that SBV induced apoptosis via both extrinsic and intrinsic pathways by activating both caspase-8 and caspase-9, respectively. Expression analyses of pro-apoptotic (Bax, Bak and Puma) and anti-apoptotic (Bcl-2 and Bcl-XL) genes revealed that SBV-induced apoptosis causes upregulation of pro-apoptotic genes, dominantly via Puma gene, whereas Bcl-2 and Bcl-XL genes were downregulated. In conclusion, this is the first detailed report about SBV induced apoptosis in the Vero cells via both extrinsic and intrinsic cascades and apoptosis induction is seem to be regulated by Puma.

【 授权许可】

Unknown